Plasma metabolic profiling analysis of toxicity induced by brodifacoum using metabonomics coupled with multivariate data analysis. (October 2016)

• Record Type: Journal Article
• Authors: Yan, Hui; Qiao, Zheng; Shen, Baohua; Xiang, Ping; Shen, Min
• Is Part Of: Forensic science international. Volume 267(2016)
• Page Start: 129
• Page End: 135
• Publication Date: 2016-10
• Subjects: Brodifacoum -- Toxicity -- Mass spectrometry -- Metabonomics -- Plasma; Medical jurisprudence -- Periodicals; Chemistry, Forensic -- Periodicals; Forensic Medicine -- Periodicals; Médecine légale -- Périodiques; Chimie légale -- Périodiques; Gerechtelijke geneeskunde; Gerechtelijke chemie; Gerechtelijke psychiatrie; Chemistry, Forensic; Medical jurisprudence; Electronic journals; Periodicals; Electronic journals; 614.1
• DOI: 10.1016/j.forsciint.2016.08.027
• Languages: English
• ISSNs: 0379-0738

Abstract

Highlights: ⿢ Metabonomic investigations were performed in plasma of rats administrated with brodifacoum. ⿢ GC⿿MS and LC⿿MS/MS were both applied to screen potential biomarkers. ⿢ Brodifacoum induces alteration in lipid metabolism and amino acid metabolism. Abstract: Brodifacoum is one of the most widely used rodenticides for rodent control and eradication; however, human and animal poisoning due to primary and secondary exposure has been reported since its development. Although numerous studies have described brodifacoum induced toxicity, the precise mechanism still needs to be explored. Gas chromatography mass spectrometry (GC⿿MS) coupled with an ultra performance liquid chromatography tandem mass spectrometry (UPLC⿿MS/MS) was applied to characterize the metabolic profile of brodifacoum induced toxicity and discover potential biomarkers in rat plasma. The toxicity of brodifacoum was dose-dependent, and the high-dose group obviously manifested toxicity with subcutaneous hemorrhage. The blood brodifacoum concentration showed a positive relation to the ingestion dose in toxicological analysis. Significant changes of twenty-four metabolites were identified and considered as potential toxicity biomarkers, primarily involving glucose metabolism, lipid metabolism and amino acid metabolism associated with anticoagulant activity, nephrotoxicity and hepatic damage. MS-based metabonomics analysis in plasma samples is helpful to search for potential poisoning biomarkers and to understand the underlying mechanisms of brodifacoum induced toxicity.