Orthogonal Sensing of Small Molecules Using a Modular Nanoparticle‐Based Assay. Issue 6 (24th March 2016)

• Record Type: Journal Article
• Authors: Neri, Simona; Pinalli, Roberta; Dalcanale, Enrico; Prins, Leonard J.
• Is Part Of: ChemNanoMat. Volume 2:Issue 6(2016)
• Page Start: 489
• Page End: 493
• Publication Date: 2016-03-24
• Subjects: chemosensing -- fluorescence -- gold nanoparticles -- indicator-displacement assay -- small molecule detection; Nanochemistry -- Periodicals; Nanostructured materials -- Periodicals; Nanochemistry; Nanostructured materials; Periodicals; 541.2
• DOI: 10.1002/cnma.201600075
• Languages: English
• ISSNs: 2199-692X

Abstract

Abstract: Herein, we present a modular indicator‐displacement assay able to selectively recognize small molecules with biological relevance under competitive conditions. The assay relies on the change in affinity of macrocyclic receptors, such as cavitands, cyclodextrins or calixarenes, for monolayer‐protected gold nanoparticles upon complexation of the respective target analyte. This change affects the equilibrium between the nanoparticles and a fluorescent reporter leading to a change in intensity of the fluorescent output signal. The recognition modules can be changed in order to tune the selectivity of the assay without affecting the nature of the output signal. The combined use of recognition modules results in an assay able to detect multiple analytes simultaneously with high selectivity. Abstract : Feel free to choose : A modular sensing assay is described that is able to detect small molecules under competitive conditions. The selectivity of the assay can be simply tuned by changing the macrocyclic receptors bound to the surface of a monolayer‐protected gold nanoparticle.