Modular protein domains: an engineering approach toward functional biomaterials. (August 2016)

• Record Type: Journal Article
• Authors: Lin, Charng-Yu; Liu, Julie C
• Is Part Of: Current opinion in biotechnology. Volume 40(2016)
• Page Start: 56
• Page End: 63
• Publication Date: 2016-08
• Subjects: Biotechnology -- Periodicals; 660.6
• DOI: 10.1016/j.copbio.2016.02.011
• Languages: English
• ISSNs: 0958-1669

Abstract

Graphical abstract: Highlights: Protein domains can be designed for specific functions. Domain functionalities include structure, bioactivity, and stimuli responsiveness. Recombinant proteins are modular and can be designed by combining functional domains. Protein domains can be added to composite materials for added functionality. Abstract : Protein domains and peptide sequences are a powerful tool for conferring specific functions to engineered biomaterials. Protein sequences with a wide variety of functionalities, including structure, bioactivity, protein–protein interactions, and stimuli responsiveness, have been identified, and advances in molecular biology continue to pinpoint new sequences. Protein domains can be combined to make recombinant proteins with multiple functionalities. The high fidelity of the protein translation machinery results in exquisite control over the sequence of recombinant proteins and the resulting properties of protein-based materials. In this review, we discuss protein domains and peptide sequences in the context of functional protein-based materials, composite materials, and their biological applications.