Isolation and expression analysis of an MAPKK gene from Fenneropenaeus chinensis in response to white spot syndrome virus infection. (August 2016)

• Record Type: Journal Article
• Authors: Li, Xupeng; Kong, Jie; Meng, Xianhong; Luo, Kun; Luan, Sheng; Cao, Baoxiang; Liu, Ning
• Is Part Of: Fish & shellfish immunology. Volume 55(2016:Aug.)
• Page Start: 116
• Page End: 122
• Publication Date: 2016-08
• Subjects: Mitogen-activated protein kinase kinase (MAPKK) -- Fenneropenaeus chinensis -- White spot syndrome virus (WSSV) -- Expression; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2016.05.004
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Mitogen-activated kinase kinase (MAPKK) is an important gene involved in the host-virus interaction process. To obtain a better understanding of MAPKK in the interaction process between the Chinese shrimp Fenneropenaeus chinensis and white spot syndrome virus (WSSV), we cloned the sequence of an MAPKK cDNA from F . chinensis ( FcMAPKK ) and investigated the effect of FcMAPKK on WSSV infection. The results showed that the FcMAPKK gene contained a 1227 bp open reading frame (ORF), which encoded a highly conserved protein with a serine/threonine protein kinase catalytic (S_TKc) domain. The deduced amino acid sequence of FcMAPKK shared identities between 11.9 and 92.6% with MAPKKs from vertebrate, invertebrate, plant and fungus species. The FcMAPKK was expressed in all the examined tissues in the normal F . chinensis . FcMAPKK expression level was highest in the hepatopancreas where it was approximately 2.6-fold the expression level in the gill, and lowest in the muscle where it was approximately 0.3-fold the expression level in the hepatopancreas. The FcMAPKK expression levels in the muscle, gill, and hepatopancreas were all changed post WSSV challenge. The FcMAPKK expression was significantly ( P < 0.01) up-regulated in the muscle of F . chinensis at 48 h post WSSV infection. The WSSV began to replicate quickly in the normal F . chinensis at 48 h post infection, while the WSSV replication in the U0126-treated F . chinensis could be significantly ( P < 0.05) inhibited. The results suggested that FcMAPKK might be involved in the WSSV infection process, and hijacking of FcMAPKK might be required for WSSV replication in F . chinensis . Highlights: We cloned the sequence of an MAPKK cDNA from Fenneropenaeus chinensis ( FcMAPKK ). The FcMAPKK gene encoded a highly conserved protein with a serine/threonine protein kinase catalytic (S_TKc) domain. FcMAPKK gene expressions in the hepatopancreas, gill and muscle were different post WSSV infection. MAPKK specific inhibitor U0126 inhibited WSSV replication significantly ( P < 0.05).