Replication of Polymer‐Based Peptide Microarrays by Multi‐Step Transfer. Issue 9 (25th July 2016)

• Record Type: Journal Article
• Authors: Striffler, Jakob; Mattes, Daniela S.; Schillo, Sebastian; Münster, Bastian; Palermo, Andrea; Ridder, Barbara; Welle, Alexander; Trouillet, Vanessa; Stadler, Volker; Markovic, Goran; Proll, Günther; Bräse, Stefan; Loeffler, Felix F.; Nesterov‐Müller, Alexander; Breitling, Frank
• Is Part Of: ChemNanoMat. Volume 2:Issue 9(2016)
• Page Start: 897
• Page End: 903
• Publication Date: 2016-07-25
• Subjects: high-throughput screening -- label-free detection -- peptide array -- replication; Nanochemistry -- Periodicals; Nanostructured materials -- Periodicals; Nanochemistry; Nanostructured materials; Periodicals; 541.2
• DOI: 10.1002/cnma.201600194
• Languages: English
• ISSNs: 2199-692X

Abstract

Abstract: Peptide microarrays serve as a high‐throughput tool for the readout of protein–peptide interactions. We present a multi‐step purification of high‐complexity peptide microarrays by transfer to specialized surfaces. Only correctly synthesized peptides are transferred from the synthesis surface to various substrates where they can be immobilized by a variety of different anchor molecules. Here, we demonstrate the transfer to rigid streptavidin, epoxide, gold, and azide surfaces. The reported method reduces the cost of purified peptide array production through the reproduction of one array several times. Abstract : Protein–peptide interaction readout : A multi‐step purification of high‐complexity peptide microarrays by transfer to specialized surfaces is presented. Only correctly synthesized peptides are transferred from the synthesis surface to various substrates where they can be immobilized by a variety of different anchor molecules, for example, streptavidin, epoxide, gold, and azide molecules.