Combined speed endurance and endurance exercise amplify the exercise‐induced PGC‐1α and PDK4 mRNA response in trained human muscle. Issue 14 (25th July 2016)

• Record Type: Journal Article
• Authors: Skovgaard, Casper; Brandt, Nina; Pilegaard, Henriette; Bangsbo, Jens
• Is Part Of: Physiological reports. Volume 4:Issue 14(2016:Jul.)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2016-07-25
• Subjects: Combined exercise -- endurance exercise -- mRNA -- muscular response -- speed endurance exercise; Physiology -- Periodicals; 571
• DOI: 10.14814/phy2.12864
• Languages: English
• ISSNs: 2051-817X

Abstract

Abstract: The aim of this study was to investigate the mRNA response related to mitochondrial biogenesis, metabolism, angiogenesis, and myogenesis in trained human skeletal muscle to speed endurance exercise ( S ), endurance exercise ( E ), and speed endurance followed by endurance exercise ( S + E ). Seventeen trained male subjects (maximum oxygen uptake ( V O2 ‐max): 57.2 ± 3.7 (mean ± SD) mL·min −1 ·kg −1 ) performed S (6 × 30 sec all‐out), E (60 min ~60% V O2 ‐max), and S + E on a cycle ergometer on separate occasions. Muscle biopsies were obtained at rest and 1, 2, and 3 h after the speed endurance exercise ( S and S + E ) and at rest, 0, 1, and 2 h after exercise in E . In S and S + E, muscle peroxisome proliferator‐activated receptor‐ γ coactivator‐1 (PGC‐1 α ) and pyruvate dehydrogenase kinase‐4 (PDK4) mRNA were higher ( P < 0.05) 2 and 3 h after speed endurance exercise than at rest. Muscle PGC‐1 α and PDK4 mRNA levels were higher ( P < 0.05) after exercise in S + E than in S and E, and higher ( P < 0.05) in S than in E after exercise. In S and S + E, muscle vascular endothelial growth factor mRNA was higher ( P < 0.05) 1 ( S only), 2 and 3 h after speed endurance exercise than at rest. In S + E, muscle regulatory factor‐4 and muscle heme oxygenase‐1 mRNA were higher ( P < 0.05) 1, 2, and 3 h after speed endurance exercise than at rest. In S, muscle hexokinase II mRNA was higher ( P < 0.05) 2 and 3 h after speed endurance exercise than at rest and higher ( P < 0.05) than in E after exercise. These findings suggest that in trained subjects, speed endurance exercise provides a stimulus for muscle mitochondrial biogenesis, substrate regulation, and angiogenesis that is not evident with endurance exercise. These responses are reinforced when speed endurance exercise is followed by endurance exercise. Abstract : Using a randomized crossover design in trained subjects, the findings suggest that speed endurance exercise provides a stimulus for muscle mitochondrial biogenesis, substrate regulation, and angiogenesis that is not evident with endurance exercise. These responses are reinforced when speed endurance exercise is followed by endurance exercise.