Label-free multiplex immunoassay of AFP, CEA and CA19-9 by integrated microfluidic biosensor based on imaging ellipsometry. Issue 171 (3rd May 2016)

• Record Type: Journal Article
• Authors: Huang, Chenghong; Yang, QingLin; Song, Fayi; Chen, Nan; Yao, Bo; Liao, Xiaoling; Zhang, ShiTong; Chen, Yanyan; Jin, Gang
• Is Part Of: Integrated ferroelectrics. Issue 171(2016)
• Page Start: 59
• Page End: 69
• Publication Date: 2016-05-03
• Subjects: Imaging ellipsometry -- immunoassay -- AFP -- CEA -- CA19-9; Ferroelectric devices -- Periodicals; Integrated circuits -- Periodicals; 537.244805
• DOI: 10.1080/10584587.2016.1171664
• Languages: English
• ISSNs: 1058-4587

Abstract

Abstract: This study describes the development of alpha-fetoprotein (AFP), carcinoembryonic antigen (CEA) and carbohydrate 19-9(CA19-9) multiplex immunoassay by integrated microfluidic device based on imaging ellipsometry (IMBIE). Different strategies for AFP, CEA and CA 19-9 simultaneous immunoassay on the same aldehyde-substrate were developed. Unifying the reaction steps of CA19-9 for antigen direct detection, CEA for secondary antibody (rabbit-antiCEA) addition, AFP for secondary (rabbit antiAFP) and an enhancer (goat IgG) application, and comprehensively considering the dynamic ranges and detection sensitivities, we combined these three markers into multiplex analysis in serial format.The sensitivities and dynamic ranges for CA19-9, CEA and AFP were 10.0U/mL, 1.0 ng/mL, 1.0 ng/mL and 2.0–100.0 U/mL, 0.1–64.0 ng/mL, 1.0–128.0 ng/mL, respectively. The intra-assay and inter-assay (coefficient variations) CVs of CA19-9, CEA and AFP were between 1.6-8.9% and 2.1–7.8%. The specificities tested for both cross-reaction and heterogeneous proteins of CA19-9, CEA and AFP were not more than 6.7%. Quantitative detection of 49 sera from gastric patients demonstrated that the accuracy for gastric cancer diagnosis was 83.4%. The total detection time is only 60 min for an automated process. The proposed assay has the potential for practical application.