Comparing Raman and fluorescence lifetime spectroscopy from human atherosclerotic lesions using a bimodal probe. Issue 9 (22nd March 2016)

• Record Type: Journal Article
• Authors: Dochow, Sebastian; Fatakdawala, Hussain; Phipps, Jennifer E.; Ma, Dinglong; Bocklitz, Thomas; Schmitt, Michael; Bishop, John W.; Margulies, Kenneth B.; Marcu, Laura; Popp, Jürgen
• Is Part Of: Journal of biophotonics. Volume 9:Issue 9(2016)
• Page Start: 958
• Page End: 966
• Publication Date: 2016-03-22
• Subjects: fluorescence lifetime -- Raman spectroscopy -- bimodal -- imaging -- fiber probes -- atherosclerosis; Photonics -- Periodicals; Optical materials -- Periodicals; Optics -- Periodicals; Medical instruments and apparatus -- Periodicals; 621.3605
• DOI: 10.1002/jbio.201500341
• Languages: English
• ISSNs: 1864-063X

Abstract

Abstract : Fluorescence lifetime imaging (FLIm) and Raman spectroscopy are two promising methods to support morphological intravascular imaging techniques with chemical contrast. Both approaches are complementary and may also be used in combination with OCT/IVUS to add chemical specificity to these morphologic intravascular imaging modalities. In this contribution, both modalities were simultaneously acquired from two human coronary specimens using a bimodal probe. A previously trained SVM model was used to interpret the fluorescence lifetime data; integrated band intensities displayed in RGB false color images were used to interpret the Raman data. Both modalities demonstrate unique strengths and weaknesses and these will be discussed in comparison to histologic analyses from the two coronary arteries imaged. Abstract : Fluorescence lifetime imaging (FLIM) and Raman are two promising methods to support morphological intravascular imaging techniques with chemical contrast. Both approaches may support each other or might be used in combination with OCT / IVUS for morphological and chemical assessment of intravascular plaques. In this contribution both modalities were simultaneously acquired from two human coronary specimens using a bimodal probe.