Stable isotope probing of carbon flow in the plant holobiont. (October 2016)

• Record Type: Journal Article
• Authors: Haichar, Feth el Zahar; Heulin, Thierry; Guyonnet, Julien P; Achouak, Wafa
• Is Part Of: Current opinion in biotechnology. Volume 41(2016)
• Page Start: 9
• Page End: 13
• Publication Date: 2016-10
• Subjects: Biotechnology -- Periodicals; 660.6
• DOI: 10.1016/j.copbio.2016.02.023
• Languages: English
• ISSNs: 0958-1669

Abstract

Graphical abstract: Stable isotope probing (SIP) approach in plant rhizosphere to analyse structure, diversity and function of active microorganisms through carbon utilisation. Highlights: Root exudation emerges as a trait involved in plant–microbe interaction. Tracking structure and functions of microbes by combining SIP and omics approaches. SIP techniques for tracing fresh and ancient carbon cycling in the rhizosphere. Use of 13 C and 15 N to evaluate nutrients transfer between plant and microbes. Abstract : Microbial communities associated with a plant host, constituting a holobiont, affect the physiology and growth of the plant via metabolites that are mainly derived from their photosynthates. The structure and function of active microbial communities that assimilate root exudates can be tracked by using stable isotope probing (SIP) approaches. This article reviews results from ongoing SIP research in plant–microbe interactions, with a specific focus on investigating the fate of fresh and recalcitrant carbon in the rhizosphere with 13 C enriched-root exudates, in addition to identifying key players in carbon cycling. Finally, we discuss new SIP applications that have the potential to identify novel enzymes implicated in rhizoremediation or plant genes dedicated to root exudation by combining SIP approaches and genome wide associations studies.