Enhanced lipid production in thermo-tolerant mutants of Chlorella pyrenoidosa NCIM 2738. (December 2016)

• Record Type: Journal Article
• Authors: Sachdeva, Neha; Gupta, Ravi Prakash; Mathur, Anshu Shankar; Tuli, Deepak Kumar
• Is Part Of: Bioresource technology. Volume 221(2016)
• Page Start: 576
• Page End: 587
• Publication Date: 2016-12
• Subjects: Microalgae -- Chemical mutagenesis -- Thermo-tolerance -- High lipid accumulation -- Dual PAM Flurometry -- Photosystem II (PSII); Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2016.09.049
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: Two thermo-tolerant mutants of Chlorella pyrenoidosa were developed. Both mutants showed >50% increase in lipid content over wildtype. Mutants could survive at temperature up to 47 °C. PAM Flurometry and cellular techniques evaluated reasons behind marked increase. Abstract: The present study aimed to develop thermo-tolerant mutants of Chlorella pyrenoidosa NCIM 2738 for high lipids production. For this, ethyl methane sulfonate was used, which generated two effective thermo-tolerant mutants, M18 and M24 of Chlorella pyrenoidosa NCIM 2738, capable of surviving at temperature up to 47 °C and showing improved lipid and biomass yields. They showed 59.62% and 50.75% increase, respectively in lipid content compared to wild type at 30 °C, which could not grow at temperature above 35 °C. The novelty of this study lied in incorporation of PAM Flurometry with mutagenesis to generate thermo-tolerant mutants of C. pyrenoidosa and investigating the reasons for increased yields of mutants at cellular and photosynthetic levels with the aim to use them for commercial biodiesel production.