Enhancement of docosahexaenoic acid production by low-energy ion implantation coupled with screening method based on Sudan black B staining in Schizochytrium sp. (December 2016)

• Record Type: Journal Article
• Authors: Fu, Jie; Chen, Tao; Lu, Hao; Lin, Yuanfeng; Xie, Xinlei; Tian, Hua; Zheng, Cao; He, Dongping
• Is Part Of: Bioresource technology. Volume 221(2016)
• Page Start: 405
• Page End: 411
• Publication Date: 2016-12
• Subjects: Schizochytrium sp. -- Low-energy ion implantation -- DHA -- Sudan black B staining -- Lipid; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2016.09.058
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: Low-energy ion implantation was applied to induce high-DHA producing mutants. Sudan black B staining method was modified for selection of targeted mutants. The maximum DHA yield of mutant strain S1 was 6.52 g/L under optimum conditions. The percentage of DHA in total lipid of S1 was 46.2% under optimum conditions. Abstract: Schizochytrium sp. is a hopeful docosahexaenoic acid (DHA) producing candidate due to its rapid growth rate and high DHA proportion in total lipid content. In this study, low-energy ion implantation was applied to Schizochytrium sp. to induce high DHA-producing mutants. Screening these mutants by Sudan black B staining, a mutant strain S1 which showed a 61% improvement in DHA production than that of the parent strain was successfully selected. Subsequently, parameters of DHA production of mutant strain S1 were optimized in a 500-mL Erlenmeyer flask. Under the optimum fermentation conditions, the production of DHA and the percentage of DHA in total lipid of mutant strain S1 were 6.52 g/L and 46.2%, respectively. This study provides an effective breeding strategy for improved DHA production of Schizochytrium sp. through combination of the novel mutagenesis technology, the effective screening method and fermentation optimization.