An auto-inducible Escherichia coli strain obtained by adaptive laboratory evolution for fatty acid synthesis from ionic liquid-treated bamboo hydrolysate. (December 2016)

• Record Type: Journal Article
• Authors: Qin, Dandan; Hu, Yuanliang; Cheng, Jie; Wang, Nan; Li, Sha; Wang, Dan
• Is Part Of: Bioresource technology. Volume 221(2016)
• Page Start: 375
• Page End: 384
• Publication Date: 2016-12
• Subjects: Adaptive laboratory evolution -- Ionic liquid -- Escherichia coli -- Fatty acids -- Bamboo hydrolysate; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2016.09.024
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: [C4 mim]Cl treated bamboo hydrolysate were explored for fatty acid production. The ALE strategy was carried out to increase ILs-tolerance of E. coli . The evolved strain could reach a final OD600 of 4.93 with 7% (w/v) ionic liquid. 2.29 g/L FFAs was produced from bamboo hydrolysate with a yield of 0.17 g/g. Abstract: Adaptive laboratory evolution (ALE) is a useful metabolic engineering strategy, which allows the selection of the microorganisms with beneficial phenotype through accumulative beneficial mutations among genetic variations occurrencely. Following ALE strategy, a rational constructed Escherichia coli strain DQ101 for fatty acids synthesis was adaptively evolved for 90 days with increasing [C4 mim]Cl concentration from 1% to 7% (w/v). The evolved strain DQ102 reached a final OD600 of 4.93 at the end of the 24 h culture with 7% (w/v) ionic liquid. DQ102/pDQTES with a thioesterase 'TesA overexpression could produce 1.12 g/L fatty acid with a productivity of 0.023 g/L-h from ionic liquid-treated bamboo hydrolysate. With another β-hydroxyacyl-ACP dehydratases ( fabZ ) overexpression, DQ102/pDQTESZ could reach a higher concentration of 2.29 g/L with a productivity of 0.048 g/L-h. These results indicated that ALE could be implemented as a useful tool for metabolic engineering and production of bio-fuels, as well as commodity and specialty chemicals.