Induced europium CPL for the selective signalling of phosphorylated amino-acids and O-phosphorylated hexapeptides. Issue 20 (25th April 2016)

• Record Type: Journal Article
• Authors: Neil, Emily R.; Fox, Mark A.; Pal, Robert; Parker, David
• Is Part Of: Dalton transactions. Volume 45:Issue 20(2016)
• Page Start: 8355
• Page End: 8366
• Publication Date: 2016-04-25
• Subjects: Chemistry, Inorganic -- Periodicals; Chemistry, Physical and theoretical -- Periodicals; Chemistry, Inorganic -- Periodicals; 546.05
• DOI: 10.1039/c6dt01212d
• Languages: English
• ISSNs: 1477-9226

Abstract

Abstract : Circularly polarised luminescence signals selectively the binding of a range of chiral phosphorylated molecules. Abstract : Two bright, europium(iii ) complexes based on an achiral heptadentate triazacyclononane ligand bearing two strongly absorbing chromophores have been evaluated for the selective emission and CPL signalling of various chiral O -phosphono-anions. Binding of O -phosphono-Ser and Thr gives rise to a strong induced CPL signature and a favoured Δ complex configuration is adopted. A similarly large induced CPL signal arises when [Eu·L 1 ] 2+ binds to lysophosphatidic acid (LPA), where the strong binding (log K 5.25 (295 K)) in methanol allowed its detection over the range 5 to 40 μM. Strong and chemoselective binding to the phosphorylated amino-acid residues was also observed with a set of four structurally related hexapeptides: in one case, the sign of the g em value in the Δ J = 1 transition allowed differentiation between the binding to O -P-Ser and O -P-Tyr residues.