Proteolytic disassembly of peptide-mediated graphene oxide assemblies for turn-on fluorescence sensing of proteases. Issue 24 (6th June 2016)

• Record Type: Journal Article
• Authors: Yang, Jin-Kyoung; Kwak, Seon-Yeong; Jeon, Su-Ji; Lee, Eunjin; Ju, Jong-Min; Kim, Hye-In; Lee, Yoon-Sik; Kim, Jong-Ho
• Is Part Of: Nanoscale. Volume 8:Issue 24(2016)
• Page Start: 12272
• Page End: 12281
• Publication Date: 2016-06-06
• Subjects: Nanoscience -- Periodicals; Nanotechnology -- Periodicals; 620.505
• DOI: 10.1039/c6nr02815b
• Languages: English
• ISSNs: 2040-3364

Abstract

Abstract : Peptide-induced assembly of fluorescent graphene oxide is developed as a 'turn-on' fluorescence biosensor for the sensitive detection of matrix metalloproteinase-2. Abstract : Molecule-induced assembly of nanomaterials can alter their unique chemical and physical properties, which can be a promising approach for sensing. Herein, we demonstrate an optical 'turn-on' biosensor for the detection of matrix metalloproteinase-2 (MMP-2), fabricated by means of a peptide-induced assembly of fluorescent graphene oxide (GO). Functionalization of GO with a peptide substrate for MMP-2 bearing a thiol group leads to its self-assembly via disulfide bonding, accompanied by self-quenching of GO's strong fluorescence. This peptide-induced GO assembly is then disassembled by proteolytic cleavage in the presence of MMP-2, thereby restoring the level of self-quenched GO fluorescence. With this approach, we are able to detect MMP-2 and to investigate the kinetic parameters of MMP-2 activity. The GO–peptide assembly is successfully applied to the selective and sensitive detection of MMP-2 secreted by living cells, human hepatocytes HepG2, at a concentration of 2 ng mL −1 .