Exploring the divalent effect in fucosidase inhibition with stereoisomeric pyrrolidine dimers. Issue 20 (3rd May 2016)

• Record Type: Journal Article
• Authors: Hottin, Audrey; Wright, Daniel W.; Moreno-Clavijo, Elena; Moreno-Vargas, Antonio J.; Davies, Gideon J.; Behr, Jean-Bernard
• Is Part Of: Organic & biomolecular chemistry. Volume 14:Issue 20(2016)
• Page Start: 4718
• Page End: 4727
• Publication Date: 2016-05-03
• Subjects: Chemistry, Organic -- Periodicals; Bioorganic chemistry -- Periodicals; Chemistry, Physical organic -- Periodicals; 547
• DOI: 10.1039/c6ob00647g
• Languages: English
• ISSNs: 1477-0520

Abstract

Abstract : The possible mechanisms of action of a dimeric fucosidase inhibitor are discussed through enzymatic assays of a series of analogues and crystallographic analysis of the enzyme-inhibitor complex. Abstract : Multi-valent inhibitors offer promise for the enhancement of therapeutic compounds across a range of chemical and biological processes. Here, a significant increase in enzyme-inhibition potencies was observed with a dimeric iminosugar-templated fucosidase inhibitor (IC50 = 0.108 μM) when compared to its monovalent equivalent (IC50 = 2.0 μM). Such a gain in binding is often attributed to a "multivalent effect" rising from alternative recapture of the scaffolded binding epitopes. The use of control molecules such as the meso analogue (IC50 = 0.365 μM) or the enantiomer (IC50 = 569 μM), as well as structural analysis of the fucosidase-inhibitor complex, allowed a detailed analysis of the possible mechanism of action, at the molecular level. Here, the enhanced binding affinity of the dimer over the monomer can be attributed to additional interactions in non-catalytic sites as also revealed in the 3-D structure of a bacterial fucosidase inhibitor complex.