Dendrimer surface orientation of the RGD peptide affects mesenchymal stem cell adhesion. Issue 55 (20th May 2016)

• Record Type: Journal Article
• Authors: Vida, Y.; Collado, D.; Najera, F.; Claros, S.; Becerra, J.; Andrades, J. A.; Perez-Inestrosa, E.
• Is Part Of: RSC advances. Volume 6:Issue 55(2016)
• Page Start: 49839
• Page End: 49844
• Publication Date: 2016-05-20
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c6ra06177j
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : Mesenchymal stem cells (MSCs) are promising candidates for a range of tissue regeneration applications. Abstract : Mesenchymal stem cells (MSCs) are promising candidates for a range of tissue regeneration applications. Adequate scaffolds are necessary for their application in vivo, where interactions between cells and the surface material are critical. Arginine-glycine-aspartic acid tripeptides (RGD) were conjugated to polyamidoamine (PAMAM) dendrimers and used to pre-treat test surfaces. We demonstrate that pre-treatment with dendrimer-presented tripeptides efficiently increases MSC adhesion to a polystyrene test surface, and that treatment effectiveness is related to how tripeptides are presented by the dendrimer to the cell. We tested both R-G-D–dendrimer and dendrimer–R-G-D arrangements and found the former to be optimal in terms of surface adhesion.