In vivo and in vitro effects of tunnel wash water and traffic related contaminants on aquatic organisms. (December 2016)

• Record Type: Journal Article
• Authors: Petersen, Karina; Bæk, Kine; Grung, Merete; Meland, Sondre; Ranneklev, Sissel Brit
• Is Part Of: Chemosphere. Volume 164(2016)
• Page Start: 363
• Page End: 371
• Publication Date: 2016-12
• Subjects: Tunnel wash water -- Algae -- Primary fish hepatocytes -- CYP1A -- Toxicity -- Chloro- and nitro-PAHs; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2016.08.108
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: In order to maintain the construction and safety of road tunnels, they are routinely washed. The wash water appears to be highly polluted with a plethora of contaminants in elevated concentrations. In addition, new and emerging compounds are likely to occur. The discharge water has shown acute toxic and sub-lethal effects in several organisms. In this study, ecotoxicity tests with algae ( Pseudokirchneriella subcapitata ) and in vitro tests with primary rainbow trout ( Oncorhynchus mykiss ) hepatocytes were used to characterize the effect of TWW from three different tunnels. In addition, selected N- and Cl-PAHs were tested for cytotoxicity, EROD activity and CYP1A protein production. TWW samples and/or extracts from two tunnels reduced the algal growth and induced cytotoxicity, EROD activity and CYP1A protein production in vitro . Four of the eight tested Cl- and N-substituted PAHs induced EROD activity and CYP1A protein production at micro-molar concentrations. N-PAHs were detected in samples from the tunnel wash, highlighting substituted PAHs as potentially important traffic-related contaminants. Highlights: Tunnel wash water (TWW) were analyzed by chemical analysis and effect studies. Metals, PAHs and N-PAHs were detected in samples from the tunnel washes. TWW had a low effect on algal growth. TWW extracts induced EROD activity and CYP1A production in primary fish hepatocytes. Two Cl- and two N-PAHs induced EROD activity and CYP1A level in primary fish hepatocytes.