Process development for the production of bioethanol from waste algal biomass of Gracilaria verrucosa. (November 2016)

• Record Type: Journal Article
• Authors: Shukla, Rishikesh; Kumar, Manoj; Chakraborty, Subhojit; Gupta, Rishi; Kumar, Savindra; Sahoo, Dinabandhu; Kuhad, Ramesh Chander
• Is Part Of: Bioresource technology. Volume 220(2016)
• Page Start: 584
• Page End: 589
• Publication Date: 2016-11
• Subjects: Algal pulp -- Biorefinery -- Bioethanol -- Fertilizer -- Gracilaria verrucosa; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2016.08.096
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: Gracilaria verrucosa, a red algae was selected for agar extraction. Left over algal pulp was used for enzymatic saccharification. Enzymatic hydrolysate was fermented to produce bioethanol. Saccharification and fermentation were successfully scaled up to bioreactor level. Abstract: The algal biomass of different species of Gracilaria were collected from coasts of Orissa and Tamil Nadu, India and characterized biochemically. Among various species, G. verrucosa was found to be better in terms of total carbohydrate content (56.65%) and hence selected for further studies. The agar was extracted from algal biomass and the residual pulp was enzymatically hydrolyzed. The optimization of algal pulp hydrolysis for various parameters revealed a maximum sugar release of 75.8 mg/ml with 63% saccharification yield. The fermentation of enzymatic hydrolysate of algal pulp was optimized and 8% (v/v) inoculum size, 12 h inoculum age, pH 5.0 were found to be optimum parameters for maximum ethanol concentration (27.2 g/L) after 12 h. The process of enzymatic hydrolysis and fermentation were successfully scaled up to 2 L bioreactor scale.