Quality of placental RNA: Effects of explant size and culture duration. (October 2016)

• Record Type: Journal Article
• Authors: Brew, O.; Nikolopoulou, E.; Hughes, A.; Christian, M.; Lee, Y.; Oduwole, O.; Sullivan, M.H.F.; Woodman, A.
• Is Part Of: Placenta. Volume 46(2016:Oct.)
• Page Start: 45
• Page End: 48
• Publication Date: 2016-10
• Subjects: Placenta -- RNA quality -- Micro explant -- Macro explants -- Explant viability -- Oxygen concentration -- Syncytial regeneration; Placenta -- Periodicals; Reproduction -- Periodicals; Placenta -- Periodicals; Placenta -- Périodiques; Reproduction -- Périodiques; 612.63
• DOI: 10.1016/j.placenta.2016.08.083
• Languages: English
• ISSNs: 0143-4004

Abstract

Abstract: We evaluated the impact of placental micro (≤50 mg) and macro (∼200 mg) explants, oxygen concentration and culture method on placental RNA quality after long-term culture. Our findings show that micro explants cultured at 8% oxygen have the best RNA quality and tissue structure. Macro explants were less viable after long-term culture. Macro explants and explants undergoing syncytial degeneration produced poor quality RNA and should be avoided. Highlights: Placental explant size (micro or macro) and duration of culture affect explant RNA quality. Micro explants (∼50 mg) in optimum long-term culture at 8% oxygen produced RNA with quality akin to pre-culture explants. Quality of RNA from explants in early cultures is compromised. Macro explants (∼200 mg) after culture produced poor quality RNA and should be avoided.