Sca‐1+mesenchymal stromal cells inhibit splenic marginal zone B lymphocytes commitment through Caspase‐3. (25th February 2016)

• Record Type: Journal Article
• Authors: Chen, Yaozhen; Yang, Jialei; Zhang, Hui‐Jie; Fan, Hong; An, Ning; Xin, Jiajia; Li, Na; Xu, Jinmei; Yin, Wen; Wu, Zhongliang; Hu, Xingbin
• Is Part Of: Cell biology international. Volume 40:Number 5(2016)
• Page Start: 549
• Page End: 559
• Publication Date: 2016-02-25
• Subjects: Caspase‐3 -- Caspase‐3 knockout mice -- HSC niche -- marginal zone B cells -- mesenchymal stromal cells -- Sca‐1+MSCs; Cytology -- Periodicals; Cells -- Periodicals; 571.605
• DOI: 10.1002/cbin.10591
• Languages: English
• ISSNs: 1065-6995

Abstract

Abstract: Mesenchymal stromal cells (MSCs) have been characterized as an important component of hematopoietic niche, which are capable of modulating the immune system through interaction with a wide range of immune cells. Marginal zone B cells, one main type of mature B lymphocytes, play a central role in eliciting antibody response against pathogens. However, how MSCs and its subpopulations regulate marginal zone B cells commitment is unknown yet. In this study, we assessed the contribution of Sca‐1 + MSCs on marginal zone B cells commitment. Our results showed that Sca‐1 + MSCs inhibit the commitment of marginal zone B lymphocytes. The inhibition was exerted through lowered Caspase‐3 expression. Furthermore, we found marginal zone B lymphocytes in spleen of Caspase‐3 knockout mice decreased and Caspase‐3 knockout Sca‐1 + MSCs accounted for the MZB lymphocytes decrease. In conclusion, our investigation provided clues about Sca‐1 + MSCs regulation on the commitment of marginal zone B cells through Caspase‐3 gene.