Preparation of biodiesel from nonedible oils using a mixture of used lipases. Issue 18 (16th September 2016) - Karmee, Sanjib Kumar
ABSTRACT: Biodiesel was synthesized from nonedible oils using a lipase mixture composed of used and discarded Candida rugusa, Candida antactica -B (Novozyme-435), Pseudomonas cepacia, Rhizopus oryzae, and porcine pancreas Type II lipase. To avoid the lipase deactivation stepwise addition of 6 mmol of methanol to 1 mmol of oil lead to 93% biodiesel yield. Addition of 10 wt% of silica gel to the reaction mixture resulted in 97% biodiesel. The lipase mixture was recycled for five times and at the end of the fifth cycle 86% biodiesel was formed.