Determination of dopamine using the fluorescence quenching of 2, 3-diaminophenazine. Issue 1 (2nd January 2017)

• Record Type: Journal Article
• Authors: Niu, Shuyan; Fang, Yajie; Zhang, Kun; Sun, Juan; Wan, Jun
• Is Part Of: Instrumentation science & technology. Volume 45:Issue 1(2017)
• Page Start: 101
• Page End: 110
• Publication Date: 2017-01-02
• Subjects: 2, 3-Diaminophenazine -- dopamine -- fluorescence quenching -- manganese; Scientific apparatus and instruments -- Periodicals; 681.2
• DOI: 10.1080/10739149.2016.1201102
• Languages: English
• ISSNs: 1073-9149

Abstract

ABSTRACT: A facile and sensitive fluorescent strategy for the determination of dopamine is reported using 2, 3-diaminophenazine. Dopamine caused autooxidation in the presence of oxygen with catalysis by Mn 2+ and formed 2, 3-dihydro-1H-indole-5, 6-dione. 2, 3-Diaminophenazine provided relatively strong fluorescence. 2, 3-Diaminophenazine in aqueous solution caused fluorescence quenching when the aminochrome condensed with 2, 3-diaminophenazine to allow the determination of dopamine. Under the optimized conditions, the linear dynamic range for dopamine was from 2.0 × 10 −6 mol · L −1 to 6.1 × 10 −5 mol · L −1 with a detection limit of 1.76 × 10 −6 mol · L −1 .