Curcumin-loaded embryonic stem cell exosomes restored neurovascular unit following ischemia-reperfusion injury. (October 2016)

• Record Type: Journal Article
• Authors: Kalani, Anuradha; Chaturvedi, Pankaj; Kamat, Pradip K.; Maldonado, Claudio; Bauer, Philip; Joshua, Irving G.; Tyagi, Suresh C.; Tyagi, Neetu
• Is Part Of: International journal of biochemistry & cell biology. Volume 79(2016:Oct.)
• Page Start: 360
• Page End: 369
• Publication Date: 2016-10
• Subjects: AchE acetylcholinestrase -- BBB blood brain barrier -- BSA bovine serum albumin -- cDNA complimentary DNA -- DAPI 4, 6-diamidino-2-phenyl-indole HCl -- FIU fluorescence intensity units -- GAPDH glyceraldehyde-3-phosphate dehydrogenase -- GFAP glial fibrillary acidic protein -- ICAM intra cellular adhesion molecule-1 -- IHC Immunohistochemistry -- IR Ischemia Reperfusion -- LEA Lycopersicon Esculentum agglutinin tomato lectin -- LPS Lipopolysaccharide -- MESC-exo Mouse embryonic stem cell exosomes -- MESC-exocur curcumin loaded mouse embryonic stem cell exosomes -- NeuN neuronal nuclei -- NMDARs N-methyl-d-aspartate receptors -- NTA nanoparticle tracking analysis -- PBS phosphate buffer saline -- QPCR quantitative PCR -- RIPA radioimmunoprecipitation assay buffer -- ROS reactive oxygen speies -- TBS tris-buffered saline -- TBS-T tris-buffered saline with Triton X-100 -- TNF-α tumor necrosis factor -- TSG101 tumor susceptibility gene101; Curcumin -- Exosomes -- Neurons -- Stroke; Biochemistry -- Periodicals; Cytology -- Periodicals; Biochemistry -- Periodicals; Cell Biology -- Periodicals; Biochimie -- Périodiques; Cytologie -- Périodiques; Biochimie; Cytologie; Biochemistry; Cytology; Ressource Internet (Descripteur de forme); Périodique électronique (Descripteur de forme); Periodicals; 572.05
• DOI: 10.1016/j.biocel.2016.09.002
• Languages: English
• ISSNs: 1357-2725

Abstract

Highlights: This is the important study that describes the combined therapeutic potentials of curcumin and embryonic stem cell exosomes. Stem cells have rejuvenating properties and curcumin possesses anti-inflammatory, anti-lipidemic and neurorestoration properties. The combined therapeutic units reduced ischemic injury and restored neuro-vascular unit following ischemia-reperfusion injury in mice. Abstract: We tested whether the combined nano-formulation, prepared with curcumin (anti-inflammatory and neuroprotective molecule) and embryonic stem cell exosomes ( MESC-exo cur ), restored neurovascular loss following an ischemia reperfusion (IR) injury in mice. IR-injury was created in 8–10 weeks old mice and divided into two groups. Out of two IR-injured groups, one group received intranasal administration of MESC-exo cur for 7 days. Similarly, two sham groups were made and one group received MESC-exo cur treatment. The study determined that MESC-exo cur treatment reduced neurological score, infarct volume and edema following IR-injury. As compared to untreated IR group, MESC-exo cur treated-IR group showed reduced inflammation and N -methyl-d -aspartate receptor expression. Treatment of MESC-exo cur also reduced astrocytic GFAP expression and alleviated the expression of NeuN positive neurons in IR-injured mice. In addition, MESC-exo cur treatment restored vascular endothelial tight (claudin-5 and occludin) and adherent (VE-cadherin) junction proteins in IR-injured mice as compared to untreated IR-injured mice. These results suggest that combining the potentials of embryonic stem cell exosomes and curcumin can help neurovascular restoration following ischemia-reperfusion injury in mice.