Expression and characterization of a lipase-related protein in the malpighian tubules of the Chinese oak silkworm, Antheraea pernyi. Issue 5 (14th June 2016)

• Record Type: Journal Article
• Authors: Wang, L.; Li, J.; Zhao, X.; Qian, C.; Wei, G.; Zhu, B.; Liu, C.
• Is Part Of: Bulletin of entomological research. Volume 106:Issue 5(2016)
• Page Start: 615
• Page End: 623
• Publication Date: 2016-06-14
• Subjects: Antheraea pernyi, -- lipase, -- immunity, -- activity; Entomology -- Periodicals; 595.7
• DOI: 10.1017/S0007485316000365
• Languages: English
• ISSNs: 0007-4853

Abstract

Abstract: Lipases are ubiquitous enzymes in nature, which play a crucial role in fat metabolism by catalyzing the hydrolysis of triacylglycerol to free fatty acids and glycerol. However, reports concerning insect lipase are rare. In this study, we studied the expression and activity of a lipase-related protein from Antheraea pernyi (ApLRP). Recombinant ApLRP was expressed in Escherichia coli cells and used to raise rabbit anti-ApLRP polyclonal antibodies. ApLRP mRNA and protein expression were abundant in the midgut and malpighian tubules, respectively. After challenge with four different microorganisms ( E. coli, Beauveria bassiana, Micrococcus luteus and nuclear polyhedrosis virus), the expression levels of ApLRP mRNA in midgut were inducted significantly compared with the control. The different pathogens induced different ApLRP gene expression patterns. The optimum temperature and pH for the enzyme's activity were 35°C and 7.0, respectively. ApLRP activity was stimulated in the presence of Mg 2+, Na +, Ca 2+ and b-mercaptoethanol; while Zn 2+, Cu 2+ and Fe 3+ inhibited its activity. Detergents such as SDS, glycerol and Tween-20 increased the lipase activity by 20–30%. Our results indicated that ApLRP might play an important role in the innate immunity of insects.