Identification of multi‐copy suppressors for endoplasmic reticulum‐mitochondria tethering proteins in Saccharomyces cerevisiae. Issue 18 (1st September 2016)

• Record Type: Journal Article
• Authors: Kojima, Rieko; Kajiura, Shu; Sesaki, Hiromi; Endo, Toshiya; Tamura, Yasushi
• Is Part Of: FEBS letters. Volume 590:Issue 18(2016)
• Page Start: 3061
• Page End: 3070
• Publication Date: 2016-09-01
• Subjects: ERMES -- mitochondria -- phospholipid -- Saccharomyces cerevisiae; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1002/1873-3468.12358
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : In yeast, the endoplasmic reticulum (ER)‐mitochondria encounter structure (ERMES) tethers the ER to mitochondria, but its primary function remains unclear. To gain insight into ERMES functions, we screened multi‐copy suppressors of the growth‐defective phenotype of mmm1 ∆ cells, which lack a core component of ERMES, and identified MCP1, MGA2, SPT23, and YGR250C (termed RIE1 ). Spt23 and Mga2 are homologous transcription factors known to activate transcription of the OLE1 gene, which encodes the fatty acid ∆9 desaturase. We found that Ole1 partially relieves the growth defects of ERMES‐lacking cells, thus uncovering a relationship between fatty acid metabolism and ERMES functions. Abstract :