Protective effect of Pycnogenol on cisplatin-induced ototoxicity in rats. (1st November 2016)

• Record Type: Journal Article
• Authors: Eryilmaz, Aylin; Eliyatkin, Nuket; Demirci, Buket; Basal, Yesim; Kurt Omurlu, Imran; Gunel, Ceren; Aktas, Safiye; Toka, Ali; Basak, Sema
• Is Part Of: Pharmaceutical biology. Volume 54:Number 11(2016:Nov.)
• Page Start: 2777
• Page End: 2781
• Publication Date: 2016-11-01
• Subjects: Antineoplastics -- antioxidants -- cochlear apoptosis; Pharmacognosy -- Periodicals; Materia medica, Vegetable -- Periodicals; 615.321
• DOI: 10.1080/13880209.2016.1177093
• Languages: English
• ISSNs: 1388-0209

Abstract

Abstract: Context: Pycnogenol ®, which is French maritime pine bark extract, is a potent antioxidant. It is used in medical conditions caused by oxidative stress. Cisplatin ( cis -diamminedichloroplatinum II) is an antineoplastic agent. However, its serious side effects such as ototoxicity limit its usage. Objective: Antioxidants can be used to prevent ototoxicity. We investigated the effect of Pycnogenol ® on cisplatin-induced ototoxicity. Materials and methods: Rats were randomly assigned to four groups of five. Distortion product-evoked otoacoustic emissions (DPOAE) test was performed for each rat. The experimental groups were as follows: Control Group, Pycnogenol ® Group: 10 mg/kg Pycnogenol ® intraperitoneally for 7 days, Cisplatin Group: intraperitoneally 15 mg/kg single injection of cisplatin on the fifth day, Cisplatin + Pycnogenol ® Group: intraperitoneally 10 mg/kg Pycnogenol ® treatment for 7 days, additionally on the fifth day, 15 mg/kg single injection of cisplatin was given. On the eighth day, DPOAE was re-performed and rats were sacrificed. Apoptosis was evaluated histopathologically. Results: Mean percentage of apoptotic cells was 1.5, 3, 30 and 11% in organ of Corti and 2, 2, 40, 15% in spiral ganglion neurons in Control Group, Pycnogenol ® Group, Cisplatin Group and Cisplatin + Pycnogenol ® Group, respectively. Cisplatin Group and Cisplatin + Pycnogenol ® Group were significantly different when compared to Control Group histopathologically both in organ of Corti and spiral ganglion neuron ( p <0.001, p = 0.019, p = 0.001, p = 0.015). DPOAE results showed that Cisplatin + Pycnogenol ® Group was significantly different when compared to Cisplatin Group at 3, 6 and 8 kHz ( p < 0.05). Conclusion: Pycnogenol protected against cisplatin ototoxicity. Also, pycnogenol is not ototoxic.