Comparison of P75NTR‐positive and ‐negative etcomesenchymal stem cell odontogenic differentiation through epithelial–mesenchymal interaction. (31st March 2016)

• Record Type: Journal Article
• Authors: Xing, Yongjun; Nie, Xin; Chen, Guoqing; Wen, Xiujie; Li, Gang; Zhou, Xia; Tian, Weidong; Liu, Luchuan
• Is Part Of: Cell proliferation. Volume 49:Number 2(2016:Apr.)
• Page Start: 185
• Page End: 194
• Publication Date: 2016-03-31
• Subjects: Cell proliferation -- Periodicals; 571.84
• DOI: 10.1111/cpr.12248
• Languages: English
• ISSNs: 0960-7722

Abstract

Abstract: Objectives: The aim of this study was to investigate differences of odonto‐differentiation between P75 ‐neurotrophin receptor (P75 ‐NTR)‐positive ectomesenchymal stem cells ( P 75 + EMSCs) and P75 ‐NTR‐negative ectomesenchymal stem cells ( P 75 − EMSCs), and their underlying mechanisms. Materials and methods: Primary cranial neural crest‐derived cells (CNC) were isolated from the first branchial arches, and P 75 + EMSCs and P 75 − EMSCs were sorted by fluorescence‐activated cell sorting. Differentiation of P 75 + EMSCs or P 75 − EMSCs into odontoblast‐like cells was induced by dental epithelial cells in vitro or in vivo . Differential gene expression profiles between P 75 + EMSCs and P 75 − EMSCs were analysed by microarray assay. Smad4‐specific small interfering RNA and activator kartogenin were used to treat the cells, to evaluate effects of Smad4 in odonto‐differentiation of P 75 + EMSCs or P 75 − EMSCs. Results: Under induction of dental epithelium conditioned medium, P 75 + EMSCs had more mineralized node formation and higher expression of Dmp1 and Dspp compared to P 75 − EMSCs. In our in vivo study, graft of P 75 + EMSCs recombination with dental epithelium showed higher expression of DMP1 and DSP. Knock‐down of Smad4 in P 75 + EMSCs significantly downregulated expression of DMP1 and DSP, while activation of Smad4 in P 75 − EMSCs by the activator kartogenin, significantly increased DSP and DMP1 expression. Conclusions: P 75 + EMSCs showed more odonto‐differentiation potential than P 75 − EMSCs both in vivo and in vitro . Smad4 played a critical role in determination of odonto‐differentiation potential of CNC‐derived EMSCs.