A Ligand That Targets CUG Trinucleotide Repeats. Issue 42 (30th August 2016)

• Record Type: Journal Article
• Authors: Li, Jinxing; Matsumoto, Jun; Bai, Li‐Ping; Murata, Asako; Dohno, Chikara; Nakatani, Kazuhiko
• Is Part Of: Chemistry. Volume 22:Issue 42(2016)
• Page Start: 14881
• Page End: 14889
• Publication Date: 2016-08-30
• Subjects: (CUG)n repeats -- RNA -- small molecules -- SPR -- translation; Chemistry -- Periodicals; 540
• DOI: 10.1002/chem.201602741
• Languages: English
• ISSNs: 0947-6539

Abstract

Abstract: The development of small molecules that can recognize specific RNA secondary and tertiary structures is currently an important research topic for developing tools to modulate gene expression and therapeutic drugs. Expanded CUG trinucleotide repeats, known as toxic RNA, capture the splicing factor MBNL1 and are causative of neurological disorder myotonic dystrophy type 1 (DM1). Herein, the rational molecular design, synthesis, and binding analysis of 2, 9‐diaminoalkyl‐substituted 1, 10‐phenanthroline (DAP), which bound to CUG trinucleotide repeats, is described. The results of melting temperature ( T m ) analyses, surface plasmon resonance (SPR) assay, and electrospray spray ionization time‐of‐flight (ESI‐TOF) mass spectrometry showed that DAP bound to r(CUG)9 but not to r(CAG)9 and r(CGG)9 . The dual luciferase assay clearly indicated DAP bound to the r(CUG) n repeat by affecting the translation in vitro. Abstract : Repeats RNA binding : The design, synthesis, and binding analysis of 2, 9‐diaminoalkyl substituted 1, 10‐phenanthroline (DAP) binding to a r(CUG)9 repeat has been described. DAP inhibited the translation by binding to r(CUG) n repeat in dual luciferase reporter assay in vitro (see figure).