Alpha‐synuclein RT‐QuIC in the CSF of patients with alpha‐synucleinopathies. Issue 10 (28th August 2016)

• Record Type: Journal Article
• Authors: Fairfoul, Graham; McGuire, Lynne I.; Pal, Suvankar; Ironside, James W.; Neumann, Juliane; Christie, Sharon; Joachim, Catherine; Esiri, Margaret; Evetts, Samuel G.; Rolinski, Michal; Baig, Fahd; Ruffmann, Claudio; Wade‐Martins, Richard; Hu, Michele T. M.; Parkkinen, Laura; Green, Alison J. E.
• Is Part Of: Annals of clinical and translational neurology. Volume 3:Issue 10(2016)
• Page Start: 812
• Page End: 818
• Publication Date: 2016-08-28
• Subjects: Nervous system -- Diseases -- Periodicals; Neurology -- Periodicals; 616.8005
• DOI: 10.1002/acn3.338
• Languages: English
• ISSNs: 2328-9503

Abstract

Abstract: We have developed a novel real‐time quaking‐induced conversion RT‐QuIC‐based assay to detect alpha‐synuclein aggregation in brain and cerebrospinal fluid from dementia with Lewy bodies and Parkinson's disease patients. This assay can detect alpha‐synuclein aggregation in Dementia with Lewy bodies and Parkinson's disease cerebrospinal fluid with sensitivities of 92% and 95%, respectively, and with an overall specificity of 100% when compared to Alzheimer and control cerebrospinal fluid. Patients with neuropathologically confirmed tauopathies (progressive supranuclear palsy; corticobasal degeneration) gave negative results. These results suggest that RT‐QuiC analysis of cerebrospinal fluid is potentially useful for the early clinical assessment of patients with alpha‐synucleinopathies.