Albumin as a carrier for NO delivery: preparation, physicochemical characterization, and interaction with gold nanoparticles. (1st December 2016)

• Record Type: Journal Article
• Authors: Luo, Ming; Boudier, Ariane; Pallotta, Arnaud; Maincent, Philippe; Vincourt, Jean-Baptiste; Leroy, Pierre
• Is Part Of: Drug development and industrial pharmacy. Volume 42:Number 12(2016:Dec.)
• Page Start: 1928
• Page End: 1937
• Publication Date: 2016-12-01
• Subjects: Citrate-stabilized gold nanoparticles -- conformational study -- serum albumin -- S-nitrosation processes -- stability; Pharmaceutical chemistry -- Periodicals; Pharmaceutical industry -- Periodicals; Drug Industry -- Periodicals; Technology, Pharmaceutical -- Periodicals; 615.05
• DOI: 10.1080/03639045.2016.1182546
• Languages: English
• ISSNs: 0363-9045

Abstract

Abstract: Background: Nitric oxide (NO) is a gaseous transmitter playing numerous physiological roles and characterized by a short half-life. Its binding to endogenous thiols increases its stability, facilitating its storage and transport. The purpose of this study was to investigate the nitrosated serum albumin (SA-SNO) and to provide a reference for its easy preparation for further use in in vitro studies. Methods: Serum albumin (SA) was S- nitrosated by reacting with (i) NaNO2 in acidic medium; (ii) different low-molecular weight S- nitrosothiols (RSNO) ( S- nitrosocysteine (CysNO), S -nitrosoglutathione (GSNO), and S, S' -dinitrosobucillamine (Buc(NO)2 )); and (iii) diethylamine NONOate (DEA/NO). SA-SNO was purified by size exclusion chromatography and the S- nitrosation site and the rate were studied by mass spectrometry and Griess–Saville assay, respectively. Then, SA-SNO was characterized by spectrofluorimetry, dynamic light scattering, and circular dichroism. Finally, SA-SNO reactivity with citrate stabilized gold nanoparticles (AuNP-citrate) was investigated via determination of NO release. Results: S -nitrosation rates of SA were 90.1 ± 3.3, 76.8 ± 2.7, 80.3 ± 3.2, 84.8 ± 5.0, and 15.4 ± 1.9% ( n = 5), when SA was reacted with acidified NaNO2, CysNO, GSNO, Buc(NO)2, and DEA/NO, respectively. The physicochemical characterization indicated that the resulting product corresponded to a mono- S -nitrosothiol (on cysteine-34), and the conformational construction remained unchanged. Stability studies showed that the NO content was preserved over 1 week. AuNP-citrate reacted with SA-SNO with increase of its hydrodynamic diameter but preservation of SNO bond. Conclusions: SA-SNO prepared and stored under the reported conditions affords a well-defined reference suitable for in vitro studies.