3D Framework DNA Origami with Layered Crossovers. Issue 41 (15th September 2016)

• Record Type: Journal Article
• Authors: Hong, Fan; Jiang, Shuoxing; Wang, Tong; Liu, Yan; Yan, Hao
• Is Part Of: Angewandte Chemie international edition. Volume 55:Issue 41(2016)
• Page Start: 12832
• Page End: 12835
• Publication Date: 2016-09-15
• Subjects: DNA nanotechnology -- DNA origami structures -- framework structures -- molecular programming -- self-assembly; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201607050
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: Designer DNA architectures with nanoscale geometric controls provide a programmable molecular toolbox for engineering complex nanodevices. Scaffolded DNA origami has dramatically improved our ability to design and construct DNA nanostructures with finite size and spatial addressability. Here we report a novel design strategy to engineer multilayered wireframe DNA structures by introducing crossover pairs that connect neighboring layers of DNA double helices. These layered crossovers (LX) allow the scaffold or helper strands to travel through different layers and can control the relative orientation of DNA helices in neighboring layers. Using this design strategy, we successfully constructed four versions of two‐layer parallelogram structures with well‐defined interlayer angles, a three‐layer structure with triangular cavities, and a 9‐ and 15‐layer square lattices. This strategy provides a general route to engineer 3D framework DNA nanostructures with controlled cavities and opportunities to design host–guest networks analogs to those produced with metal organic frameworks. Abstract : DNA framework structures : A novel layered crossover motif is introduced to design a new kind of framework DNA origami structures (see picture). The method expands the rule of design methodology of DNA origami structures and offers new opportunities for future applications.