Live and inactivated vaccine regimens against caecal Salmonella Typhimurium colonisation in laying hens. Issue 10 (27th September 2016)

• Record Type: Journal Article
• Authors: Groves, PJ; Sharpe, SM; Muir, WI; Pavic, A; Cox, JM
• Is Part Of: Australian veterinary journal. Volume 94:Issue 10(2016)
• Page Start: 387
• Page End: 393
• Publication Date: 2016-09-27
• Subjects: layer chickens -- poultry -- Salmonella Typhimurium -- vaccines; Veterinary medicine -- Australia -- Periodicals; Veterinary medicine -- Periodicals; Veterinary Medicine -- Periodicals; 636.089
• DOI: 10.1111/avj.12490
• Languages: English
• ISSNs: 0005-0423

Abstract

Abstract : Objective: In Australia, Salmonella serovar Typhimurium ( S . Typhimurium) is the predominant zoonotic serovar in humans and is frequently isolated from layer hens. Vaccination against this serovar has been previously shown to be effective in broilers and the aim of this current study was to assess and determine the best vaccination strategy (live or inactivated) to minimise caecal colonisation by S . Typhimurium. Methods: A long‐term experiment (56 weeks) was conducted on ISABROWN pullets using a commercial live aro A deleted mutant S . Typhimurium vaccine and an autogenous inactivated multivalent Salmonella vaccine (containing serovars Typhimurium, Infantis, Montevideo and Zanzibar). These vaccines were administered PO or by SC or IM injection, either alone or in combination. Pullets were vaccinated throughout rearing (to 18 weeks of age) and sequentially bled for antibody titre levels. The birds, vaccinated and controls, were challenged orally with a field isolate of S . Typhimurium at different ages, held for 21 days post‐challenge, then euthanased and their caeca cultured for the presence of Salmonella. Results: None of the oral live‐vaccinated groups exhibited lasting protection. When administered twice, the inactivated vaccine gave significant protection at 17 weeks of age and the live vaccine given by SC injection given twice produced significant protection at 17, 25 and 34 weeks. Conclusions: Vaccination regimens that included parenteral administration of live or inactivated vaccines and thus achieved positive serum antibody levels were able to provide protection against challenge. Hence, vaccination may play a useful role in a management strategy for Salmonella carriage in layer flocks.