Preeclampsia/Eclampsia candidate genes show altered methylation in maternal leukocytes of preeclamptic women at the time of delivery. (2nd July 2016)

• Record Type: Journal Article
• Authors: White, Wendy M.; Sun, Zhifu; Borowski, Kristi S.; Brost, Brian C.; Davies, Norman P.; Rose, Carl H.; Garovic, Vesna D.
• Is Part Of: Hypertension in pregnancy. Volume 35:Number 3(2016)
• Page Start: 394
• Page End: 404
• Publication Date: 2016-07-02
• Subjects: AGT -- CALCA -- candidate gene -- DDAH1 -- DNA methylation -- leukocyte -- POMC -- preeclampsia -- pregnancy; Hypertension in pregnancy -- Periodicals; Pregnancy -- Complications -- Periodicals; 618.3261
• DOI: 10.3109/10641955.2016.1162315
• Languages: English
• ISSNs: 1064-1955

Abstract

ABSTRACT: Objective : To analyze methylation profiles of known preeclampsia/eclampsia (PE) candidate genes in normal (NL) and preeclamptic (PE) women at delivery. Methods : A matched case–control study comparing methylation in 79 CpG sites/33 genes from an independent gene set in maternal leukocyte DNA in PE and NL ( n = 14 each) on an Illumina BeadChip platform. Replication performed on second cohort (PE = 12; NL = 32). Results : PE demonstrates differential methylation in POMC, AGT, CALCA, and DDAH1 compared with NL. Conclusion : Differential methylation in four genes associated with PE may represent a potential biomarker or an epigenetic pathophysiologic mechanism altering gene transcription.