Highly selective ratiometric peptide-based chemosensors for zinc ions and applications in living cell imaging: a study for reasonable structure design. Issue 36 (30th August 2016)

• Record Type: Journal Article
• Authors: Yang, Jingchang; Rong, Huan; Shao, Ping; Tao, Yanduo; Dang, Jun; Wang, Peng; Ge, Yushu; Wu, Jiang; Liu, Dan
• Is Part Of: Journal of materials chemistry. Volume 4:Issue 36(2016)
• Page Start: 6065
• Page End: 6073
• Publication Date: 2016-08-30
• Subjects: Materials -- Periodicals; Chemistry, Analytic -- Periodicals; Biomedical materials -- Research -- Periodicals; 543.0284
• DOI: 10.1039/c6tb01570k
• Languages: English
• ISSNs: 2050-750X

Abstract

Abstract : The relationship between the structure and activity for the fluorescent sensing of zinc ions with H2 L peptides has been studied experimentally and computationally. Abstract : Recently, fluorescent peptide-based chemosensors have been reported and used to detect series of metal ions in both aqueous solutions and living cells. In order to explore and indicate the structural regulations of "fluorophore-(His) n -Pro-Gly-(His) n -fluorophore (H2 L peptide sensor)", we predict the relationship between the number of histidine molecules and the coordination activity, as well as the preferred coordination site, through computational studies. L n =3 was modeled as showing the highest activity with the lowest calculated Δ E total, and inner histidine residues bonding with the "Pro-Gly" core structure always provided essential coordination sites for zinc ions. A predicted structural regulation mechanism was studied and verified experimentally. Three Zn 2+ sensors with the number of histidine molecules set as 2 (L n =2 ), 3 (L n =3 ) and 4 (L n =4 ) were synthesized and tested. The L n =3 sensor shows the highest fluorescence intensity by specific binding with Zn 2+ . The key coordination site was identified by mutating the histidine residues of the L n =3 sensor. The inner histidine bonding with the "Pro-Gly" core structure was proved as the vital coordination site, and at least one extra histidine had to be provided to assist the coordination process according to the experimental results. Both computational and experimental studies reveal the same relationship between the structure and activity for H2 L peptides in the fluorescent sensing of zinc ions. The results give fundamental information for the reasonable design and optimization of all similar classes of peptide sensors.