Electrospinning induced surface activation (EISA) of highly porous PMMA microfiber mats for HIV diagnosis. Issue 36 (22nd August 2016) - Serafim, B. M.
Abstract : Schematic of biological coupling to both dry substrate (DS) and wet substrate (WS) deposited fibers. (A) DS fibers without coupling, (B) WS fibers coupled to AbPE and GFP fluorescent proteins, (C) WS fibers assay coupled to HIV. Abstract : This work presents an Electrospray Induced Surface Activation (EISA) method generalization for electrospinning. It allows an easy way to produce surface functionalized microfiber mats for infectious disease diagnostic purposes. We present the details of both the production and characterization of surface functionalized highly porous poly methyl methacrylate (PMMA) microfiber mats produced using dry (DS) and wet substrate (WS) configurations. The characterization was performed using high-resolution scanning electron microscopy (HRSEM), Size Exclusion Chromatography (SEC), X-ray photoelectron spectroscopy (XPS) and biological essays attaching both the recombinant auto-fluorescent green fluorescent protein (GFP) and the anti-human Ig protein containing a fluorescent reporter R-phycoerythrin (AbPE). The final biological application assay was performed by positively detecting HIV contaminated human samples.