Antimicrobial activity of hydroxytyrosol: a current controversy. Issue 4 (2nd April 2016)

• Record Type: Journal Article
• Authors: Medina-Martínez, María S.; Truchado, Pilar; Castro-Ibáñez, Irene; Allende, Ana
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 80:Issue 4(2016)
• Page Start: 801
• Page End: 810
• Publication Date: 2016-04-02
• Subjects: olive -- Escherichia coli -- antimicrobial -- growth inhibition -- kinetic parameters; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2015.1116924
• Languages: English
• ISSNs: 0916-8451

Abstract

Abstract : This study focus on the main factors that affect the antimicrobial capacity of hydroxytyrosol, including the concentration (200, 400, and 1000 μg/mL), target strains, and the culture media (nutrient-rich and less-rich culture media). The potential HT degradation was also evaluated by HPLC-PAD. Kinetic parameters from growth curves showed that HT concentrations produced a doses-dependent shift when compared to the untreated control. In most of the cases, the highest tested dose (1000 μg/mL) was needed to inhibit growth of the selected strains. However, all the strains were able to grow even at the highest HT dose when cultivated in nutrient-rich culture media. It was observed that HT concentrations were reduced by about 15%, except for Escherichia coli 533 and 679 in Muller Hinton broth, where HT was reduced up to 35%. The results showed a limited antimicrobial activity, contrary to information previously published in some research papers. Abstract :