In vitro antibiofilm activity of the freshwater bryozoan Hyalinella punctata: a case study of Pseudomonas aeruginosa PAO1. Issue 16 (17th August 2016)

• Record Type: Journal Article
• Authors: Pejin, Boris; Ciric, Ana; Karaman, Ivo; Horvatovic, Mladen; Glamoclija, Jasmina; Nikolic, Milos; Sokovic, Marina
• Is Part Of: Natural product research. Volume 30:Issue 16(2016)
• Page Start: 1847
• Page End: 1850
• Publication Date: 2016-08-17
• Subjects: Bryozoa -- extracts -- antibiofilm activity -- antiquorum sensing activity; Natural products -- Periodicals; Natural products -- Research -- Periodicals; Biological Factors -- Periodicals; Biological Products -- Periodicals; 547
• DOI: 10.1080/14786419.2015.1072714
• Languages: English
• ISSNs: 1478-6419

Abstract

Abstract: The antibiofilm and possible antiquorum sensing effects against the strain Pseudomonas aeruginosa PAO1 of five crude extracts of the freshwater bryozoan Hyalinella punctata (Hancock, 1850) were evaluated in vitro for the first time. H. punctata ethyl acetate extract (HpEtAc) exhibited the highest antibiofilm activity reducing the biofilm formation of P. aeruginosa PAO1 in the range of 80.63–88.13%. While all tested extracts reduced the twitching motility of the aforementioned bacterial strain, HpEtAc showed to be the most effective. Finally, at a concentration of 0.5 MIC, the same extract mostly inhibited the production of pyocyanin by P. aeruginosa PAO1 (71.53%). In comparison both with the positive controls used (streptomycin and ampicillin, 67.13 and 69.77%, respectively), HpEtAc was found to inhibit pyocyanin in a higher extent. An extensive chemical characterisation of this particular extract may result in isolation and identification of novel lead compounds targeting P. aeruginosa, an opportunistic human pathogen. Graphical abstract: