Development and validation of a liquid chromatography with tandem mass spectrometry method for the determination of isomangiferin in rat plasma with application to a preclinical pharmacokinetic study. Issue 15 (4th July 2016)

• Record Type: Journal Article
• Authors: Sun, Fengjun; Liu, Zhirui; Yang, Bo; Xia, Peiyuan
• Is Part Of: Journal of separation science. Volume 39:Issue 15(2016)
• Page Start: 2890
• Page End: 2895
• Publication Date: 2016-07-04
• Subjects: Isomangiferin -- Liquid chromatography with tandem mass spectrometry -- Pharmacokinetics -- Rat plasma; Separation (Technology) -- Periodicals; Chromatographic analysis -- Periodicals; 543.089
• DOI: 10.1002/jssc.201501408
• Languages: English
• ISSNs: 1615-9306

Abstract

Abstract : A sensitive and specific liquid chromatography with tandem mass spectrometry method was firstly developed for the measurement of isomangiferin in rat plasma. Chloramphenicol was selected as the internal standard. Sample preparation was carried out through a simple one‐step protein precipitation procedure with methanol. Negative electrospray ionization was performed using multiple reaction monitoring mode with transitions of m / z 421.1/301.1 for isomangiferin, and 321.1/151.9 for chloramphenicol. The calibration curve was linear over the range of 0.1–600 ng/mL, with a lower limit of quantification at 0.1 ng/mL. The intra‐ and interday precisions (relative standard deviation) were no more than 8.2% and accuracies (relative error) were within the range of –8.4 to 2.2%. The recovery, matrix effect and stability under different conditions were all proved acceptable. The validated method has been successfully applied to a preclinical pharmacokinetic study of isomangiferin in rats for the first time.