Effects of dietary lipid level on growth, fatty acid composition, digestive enzymes and expression of some lipid metabolism related genes of orange‐spotted grouper larvae (Epinephelus coioides H.). (22nd January 2015)

• Record Type: Journal Article
• Authors: Li, Songlin; Mai, Kangsen; Xu, Wei; Yuan, Yuhui; Zhang, Yanjiao; Zhou, Huihui; Ai, Qinghui
• Is Part Of: Aquaculture research. Volume 47:Number 8(2016)
• Page Start: 2481
• Page End: 2495
• Publication Date: 2015-01-22
• Subjects: dietary lipid -- growth -- digestive enzyme -- fatty acids -- gene expression -- Epinephelus coioides; Aquaculture -- Periodicals; Fishery management -- Periodicals; 639.8
• DOI: 10.1111/are.12697
• Languages: English
• ISSNs: 1355-557X

Abstract

Abstract: A 4‐week study was conducted to investigate the effects of dietary lipid level on growth, fatty acid composition, activities of digestive enzymes and some lipid metabolism related genes of orange‐spotted grouper larvae ( Epinephelous coioides ). Six isonitrogenous (57% crude protein) diets were formulated to contain graded contents of lipid (5.94%, 9.92%, 13.30%, 17.71% and 21.87% dry weight, respectively) using fish oil as main lipid source. Each diet was fed six times daily to triplicate groups of fish (71 ± 2 mg, 29 DAH). Results showed that the highest SGR and survival of larvae occurred in 17.71% and 13.30% lipid group respectively. The optimal dietary lipid requirement of larval grouper was estimated to be 15.99% based on SGR. Activities of pepsin and lipase were significantly higher in 17.71% lipid group compared to the rest ones. However, the highest activities of trypsin and amylase occurred in 9.92% and 5.94% lipid treatment respectively. The expression of fatty acid synthesis (FAS) decreased significantly with increasing dietary lipid, while the expression of lipoprotein lipase (LPL) and hormone sensitive lipase (HSL) significantly increased first and then decreased significantly. Compared to other treatment, larvae fed diets with 13.30% and 17.71% lipid have a significant higher relative expression of LPL and HSL respectively. Results of this study suggested that larvae fed the diet with appropriate lipid content showed higher growth performance. Moreover, dietary lipid content could also influence both de novo lipogenesis and lipolysis in transcriptional level.