The contribution of adenines in the catalytic core of 10-23 DNAzyme improved by the 6-amino group modifications. Issue 18 (15th September 2016)

• Record Type: Journal Article
• Authors: Zhu, Junfei; Li, Zhiwen; Wang, Qi; Liu, Yang; He, Junlin
• Is Part Of: Bioorganic & medicinal chemistry letters. Volume 26:Issue 18(2016)
• Page Start: 4462
• Page End: 4465
• Publication Date: 2016-09-15
• Subjects: 10-23 DNAzyme -- Amino group -- Imidazolyl group -- Chemical modification -- Adenine; Bioorganic chemistry -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1016/j.bmcl.2016.07.076
• Languages: English
• ISSNs: 0960-894X

Abstract

Graphical abstract: Abstract: In the catalytic core of 10-23 DNAzyme, its five adenine residues are moderate conservative, but with highly conserved functional groups like 6-amino group and 7-nitrogen atom. It is this critical conservation that these two groups could be modified for better contribution. With 2′-deoxyadenosine analogues, several functional groups were introduced at the 6-amino group of the five adenine residues. 3-Aminopropyl substituent at 6-amino group of A15 resulted in a five-fold increase of k obs . More efficient DNAzymes are expected by delicate design of the linkage and the external functional groups for this 6-amino group of A15. With this modification approach, other functional groups or residues could be optimized for 10-23 DNAzyme.