Label-free electrochemical detection of malaria-infected red blood cells. Issue 79 (10th August 2016)

• Record Type: Journal Article
• Authors: Kumar, Binod; Bhalla, Vijayender; Singh Bhadoriya, Ravi Pratap; Suri, C. Raman; Varshney, Grish C.
• Is Part Of: RSC advances. Volume 6:Issue 79(2016)
• Page Start: 75862
• Page End: 75869
• Publication Date: 2016-08-10
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c6ra07665c
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : The precise and rapid diagnosis of malaria is key to prevent indiscriminate use of antimalarial drugs and help in timely treatment and management of the disease. Abstract : The precise and rapid diagnosis of malaria is key to prevent indiscriminate use of antimalarial drugs and help in timely treatment and management of the disease. This paper reports a label-free detection of P. falciparum infected red blood cells using a gold nanoparticle (GNP) enhanced platform. The GNPs were electrodeposited on screen-printed electrodes to form a well-controlled matrix that served the dual role of antibody immobilization and signal enhancement. The detection of infected red blood cells was carried out by measuring changes in electrical parameters as a result of its binding to cell reactive antibodies immobilized on the electrode. The assay showed good sensitivity and a linear response between the electron transfer resistance and the logarithm of the number of infected red blood cells which was observed over a concentration range of 10 2 cells per mL to 10 8 cells per mL. This is the first report where an antibody-functionalized electrochemical biosensing platform has been employed for the quantitative detection of P. falciparum infected whole red blood cells.