Detection of Isoforms Differing by a Single Charge Unit in Individual Cells. (6th September 2016)
- Record Type:
- Journal Article
- Title:
- Detection of Isoforms Differing by a Single Charge Unit in Individual Cells. (6th September 2016)
- Main Title:
- Detection of Isoforms Differing by a Single Charge Unit in Individual Cells
- Authors:
- Tentori, Augusto M.
Yamauchi, Kevin A.
Herr, Amy E. - Abstract:
- Abstract: To measure protein isoforms in individual mammalian cells, we report single‐cell resolution isoelectric focusing (scIEF) and high‐selectivity immunoprobing. Microfluidic design and photoactivatable materials establish the tunable pH gradients required by IEF and precisely control the transport and handling of each 17‐pL cell lysate during analysis. The scIEF assay resolves protein isoforms with resolution down to a single‐charge unit, including both endogenous cytoplasmic and nuclear proteins from individual mammalian cells.
- Is Part Of:
- Angewandte Chemie. Volume 128:Number 40(2016)
- Journal:
- Angewandte Chemie
- Issue:
- Volume 128:Number 40(2016)
- Issue Display:
- Volume 128, Issue 40 (2016)
- Year:
- 2016
- Volume:
- 128
- Issue:
- 40
- Issue Sort Value:
- 2016-0128-0040-0000
- Page Start:
- 12619
- Page End:
- 12623
- Publication Date:
- 2016-09-06
- Subjects:
- Einzelzellanalyse -- Immunassays -- Isoelektrische Fokussierung -- Lab-on-a-Chip -- Proteomik
Chemistry -- Periodicals
540 - Journal URLs:
- http://onlinelibrary.wiley.com/ ↗
- DOI:
- 10.1002/ange.201606039 ↗
- Languages:
- English
- ISSNs:
- 0044-8249
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - 0902.000000
British Library DSC - BLDSS-3PM
British Library HMNTS - ELD Digital store - Ingest File:
- 64.xml