Laboratory course on Streptomyces genetics and secondary metabolism. Issue 5 (18th May 2016)

• Record Type: Journal Article
• Authors: Siitonen, Vilja; Räty, Kaj; Metsä‐Ketelä, Mikko
• Is Part Of: Biochemistry and molecular biology education. Volume 44:Issue 5(2016:Sep./Oct.)
• Page Start: 492
• Page End: 499
• Publication Date: 2016-05-18
• Subjects: Streptomyces -- nogalamycin -- rhodomycin -- secondary metabolites; Biochemistry -- Study and teaching -- Periodicals; Molecular biology -- Study and teaching -- Periodicals; 572.071
• DOI: 10.1002/bmb.20970
• Languages: English
• ISSNs: 1470-8175

Abstract

Abstract: The " Streptomyces genetics and secondary metabolism" laboratory course gives an introduction to the versatile soil dwelling Gram‐positive bacteria Streptomyces and their secondary metabolism. The course combines genetic modification of Streptomyces ; growing of the strain and protoplast preparation, plasmid isolation by alkaline lysis and phenol precipitation, digestions, and ligations prior to protoplast transformation, as well as investigating the secondary metabolites produced by the strains. Thus, the course is a combination of microbiology, molecular biology, and chemistry. After the course the students should understand the relationship between genes, proteins, and the produced metabolites. © 2016 by The International Union of Biochemistry and Molecular Biology, 44(5):492–499, 2016.