Multimodal nonlinear microscopy of head and neck carcinoma — toward surgery assisting frozen section analysis. Issue 10 (21st April 2016)

• Record Type: Journal Article
• Authors: Heuke, Sandro; Chernavskaia, Olga; Bocklitz, Thomas; Legesse, Fisseha Bekele; Meyer, Tobias; Akimov, Denis; Dirsch, Olaf; Ernst, Günther; von Eggeling, Ferdinand; Petersen, Iver; Guntinas–Lichius, Orlando; Schmitt, Michael; Popp, Jürgen
• Is Part Of: Head & neck. Volume 38:Issue 10(2016)
• Page Start: 1545
• Page End: 1552
• Publication Date: 2016-04-21
• Subjects: nonlinear microscopy -- spectral histopathology -- head and neck cancer imaging -- coherent anti‐Stokes Raman scattering (CARS) -- second‐harmonic generation (SHG) -- two‐photon excited fluorescence (TPEF) -- image analysis; Head -- Diseases -- Periodicals; Neck -- Diseases -- Periodicals; Head -- Periodicals; Neck -- Periodicals; Face -- Periodicals; 617.51059
• DOI: 10.1002/hed.24477
• Languages: English
• ISSNs: 1043-3074

Abstract

Abstract: Background: Treatment of early cancer stages is deeply connected to a good prognosis, a moderate reduction of the quality of life, and comparably low treatment costs. Methods: Head and neck squamous cell carcinomas were investigated using the multimodal combination of coherent anti‐Stokes Raman scattering (CARS), two‐photon excited fluorescence (TPEF), and second‐harmonic generation (SHG) microscopy. Results: An increased median TPEF to CARS contrast was found comparing cancerous and healthy squamous epithelium with a p value of 1.8·10 −10 . A following comprehensive image analysis was able to predict the diagnosis of imaged tissue sections with an overall accuracy of 90% for a 4‐class model. Conclusion: Nonlinear multimodal imaging is verified objectively as a valuable diagnostic tool that complements conventional staining protocols and can serve as filter in future clinical routine reducing the pathologist's workload. © 2016 Wiley Periodicals, Inc. Head Neck 38: First–1552, 2016