Rational design of novel high molecular weight solubilization surfactants for membrane proteins from the peptide gemini surfactants (PG-surfactants). Issue 43 (27th October 2016)

• Record Type: Journal Article
• Authors: Koeda, Shuhei; Suzuki, Tomoyuki; Noji, Tomoyasu; Kawakami, Keisuke; Itoh, Shigeru; Dewa, Takehisa; Kamiya, Nobuo; Mizuno, Toshihisa
• Is Part Of: Tetrahedron. Volume 72:Issue 43(2016)
• Page Start: 6898
• Page End: 6908
• Publication Date: 2016-10-27
• Subjects: Solubilization surfactant -- Membrane protein -- Gemini surfactant -- Photosystem I -- Photosystem II; Chemistry, Organic -- Periodicals; 547.005
• DOI: 10.1016/j.tet.2016.09.024
• Languages: English
• ISSNs: 0040-4020

Abstract

Abstract: We successfully developed novel high molecular weight (Mw) solubilization surfactants for membrane proteins, Bis-D 3 -DKDKC 12 (Mw ∼3 kDa), Bis-K 3 -DKDKC 12 (Mw ∼3 kDa), andTris-D 3 -DKDKC 12 (Mw ∼4.3 kDa), which were rationally designed from our previously established peptide gemini surfactants (PG-surfactants)DKDKC 12 K andDKDKC 12 D (Mw ∼1.3 kDa). An increase in Mw enhanced affinity between membrane proteins and surfactants, thereby allowing effective solubilization, even for lower concentration ranges (<0.0005 wt %). Graphical abstract: