An exon skipping in a SEPALLATA‐Like gene is associated with perturbed floral and fruits development in cucumber. Issue 9 (20th April 2016)

• Record Type: Journal Article
• Authors: Wang, Xin; Gao, Dongli; Sun, Jinjing; Liu, Min; Lun, YaoYao; Zheng, Jianshu; Wang, Shenhao; Cui, Qingzhi; Wang, Xiaofeng; Huang, Sanwen
• Is Part Of: Journal of integrative plant biology. Volume 58:Issue 9(2016)
• Page Start: 766
• Page End: 771
• Publication Date: 2016-04-20
• Subjects: Cucumis sativus L -- EMS mutant -- exon skipping -- floral and fruits development -- whole genome re‐sequencing; Plants -- Periodicals; Plants -- China -- Periodicals; Electronic journals; 580.5
• DOI: 10.1111/jipb.12472
• Languages: English
• ISSNs: 1672-9072

Abstract

Summary: We isolated a mutant showing perturbations in the development of male and female floral organs and fruits. Analysis of the single nucleotide polymorphisms from bulked F2 pools identified the causative variant occurring in Csa4G126690 . Csa4G126690 shows high homology to Arabidopsis SEPALLATA2 ( SEP2 ) thus being designated CsSEP2 . The causative variant was located on the splicing site of CsSEP2, resulting in the skipping of exon 6 and abolishment of the transcriptional activity. Our data suggest that CsSEP2 is involved in the floral organ and fruits development by conferring transcriptional activity. Abstract : We isolated a mutant showing perturbations in the development of floral organs and fruits in cucumber. We further identified the causative variant occurring in CsSEP2 by whole genome re‐sequencing of the mutant. The variant caused the skipping of exon 6 and abolishment of the transcriptional activity, thus perturbing floral organs and fruits development.