Automated nuclei segmentation of malignant using level sets. Issue 10 (1st August 2016)

• Record Type: Journal Article
• Authors: Husham, Ahmed; Hazim Alkawaz, Mohammed; Saba, Tanzila; Rehman, Amjad; Saleh Alghamdi, Jarallah
• Is Part Of: Microscopy research and technique. Volume 79:Issue 10(2016:Oct.)
• Page Start: 993
• Page End: 997
• Publication Date: 2016-08-01
• Subjects: histopathology -- level sets -- malignant detection -- nuclei -- segmentation; Electron microscopy -- Technique -- Periodicals; Microscopy -- Periodicals; Microscopy -- Technique -- Periodicals; 502.825
• DOI: 10.1002/jemt.22733
• Languages: English
• ISSNs: 1059-910X

Abstract

Abstract: Segmentation of objects from a noisy and complex image is still a challenging task that needs to be addressed. This article proposed a new method to detect and segment nuclei to determine whether they are malignant or not (determination of the region of interest, noise removal, enhance the image, candidate detection is employed on the centroid transform to evaluate the centroid of each object, the level set [LS] is applied to segment the nuclei). The proposed method consists of three main stages: preprocessing, seed detection, and segmentation. Preprocessing stage involves the preparation of the image conditions to ensure that they meet the segmentation requirements. Seed detection detects the seed point to be used in the segmentation stage, which refers to the process of segmenting the nuclei using the LS method. In this research work, 58 H&E breast cancer images from the UCSB Bio‐Segmentation Benchmark dataset are evaluated. The proposed method reveals the high performance and accuracy in comparison to the techniques reported in literature. The experimental results are also harmonized with the ground truth images.