Low-intensity repetitive magnetic stimulation lowers action potential threshold and increases spike firing in layer 5 pyramidal neurons in vitro. (29th October 2016)

• Record Type: Journal Article
• Authors: Tang, Alexander D.; Hong, Ivan; Boddington, Laura J.; Garrett, Andrew R.; Etherington, Sarah; Reynolds, John N.J.; Rodger, Jennifer
• Is Part Of: Neuroscience. Volume 335(2016)
• Page Start: 64
• Page End: 71
• Publication Date: 2016-10-29
• Subjects: ACSF artificial cerebrospinal fluid -- AHP after hyperpolarization -- AP action potential -- LI-rMS low-intensity repetitive magnetic stimulation -- RMP resting membrane potential -- rTMS repetitive transcranial magnetic stimulation; low-intensity rMS -- action potential threshold -- spike firing frequency -- Intermittent Theta Burst Stimulation; Neurochemistry -- Periodicals; Neurophysiology -- Periodicals; Neurology -- Periodicals; Neurochimie -- Périodiques; Neurophysiologie -- Périodiques; Neurochemistry; Neurophysiology; Electronic journals; Periodicals; Electronic journals; 612.8
• DOI: 10.1016/j.neuroscience.2016.08.030
• Languages: English
• ISSNs: 0306-4522

Abstract

Highlights: The acute effect of LI-rMS on layer 5 pyramidal neurons was investigated in vitro . LI-rMS does not alter passive membrane properties. LI-rMS induced hyperpolarized action potential thresholds. LI-rMS Increased evoked spike firing frequency. Abstract: Repetitive transcranial magnetic stimulation (rTMS) has become a popular method of modulating neural plasticity in humans. Clinically, rTMS is delivered at high intensities to modulate neuronal excitability. While the high-intensity magnetic field can be targeted to stimulate specific cortical regions, areas adjacent to the targeted area receive stimulation at a lower intensity and may contribute to the overall plasticity induced by rTMS. We have previously shown that low-intensity rTMS induces molecular and structural plasticity in vivo, but the effects on membrane properties and neural excitability have not been investigated. Here we investigated the acute effect of low-intensity repetitive magnetic stimulation (LI-rMS) on neuronal excitability and potential changes on the passive and active electrophysiological properties of layer 5 pyramidal neurons in vitro . Whole-cell current clamp recordings were made at baseline prior to subthreshold LI-rMS (600 pulses of iTBS, n = 9 cells from 7 animals) or sham ( n = 10 cells from 9 animals), immediately after stimulation, as well as 10 and 20 min post-stimulation. Our results show that LI-rMS does not alter passive membrane properties (resting membrane potential and input resistance) but hyperpolarises action potential threshold and increases evoked spike-firing frequency. Increases in spike firing frequency were present throughout the 20 min post-stimulation whereas action potential (AP) threshold hyperpolarization was present immediately after stimulation and at 20 min post-stimulation. These results provide evidence that LI-rMS alters neuronal excitability of excitatory neurons. We suggest that regions outside the targeted region of high-intensity rTMS are susceptible to neuromodulation and may contribute to rTMS-induced plasticity.