A CgIFNLP receptor from Crassostrea gigas and its activation of the related genes in human JAK/STAT signaling pathway. (December 2016)

• Record Type: Journal Article
• Authors: Zhang, Ran; Liu, Rui; Xin, Lusheng; Chen, Hao; Li, Chenghua; Wang, Lingling; Song, Linsheng
• Is Part Of: Developmental and comparative immunology. Volume 65(2016)
• Page Start: 98
• Page End: 106
• Publication Date: 2016-12
• Subjects: Crassostrea gigas -- Interferon -- Interferon receptor -- Poly (I: C) -- Interferon stimulated genes; Immunology -- Periodicals; Developmental immunology -- Periodicals; 616.079
• DOI: 10.1016/j.dci.2016.06.010
• Languages: English
• ISSNs: 0145-305X

Abstract

Abstract: Interferon is a highly pleiotropic cytokine, once binding to its receptors, can activate JAK kinases and STAT transcription factors to initiate the transcription of genes downstream from interferon-stimulated response elements. In the present study, a cytokine receptor-like 3 molecule was selected and cloned from oyster Crassostrea gigas, which contained a fibronectin type III domain (designed Cg IFNR-3). The expression pattern of Cg IFNR-3 mRNA was detected in all the tested tissues including mantle, gills, hepatopancreas, muscle, and hemocytes, with the highest expression level in hemocytes. After poly (I: C) stimulation, the expression level of Cg IFNR-3 in hemocytes was observed to significantly increase at 3 h (13.06-fold, p < 0.01). Cg IFNR-3 was indicated to interact with Cg IFNLP by in vitro GST pull-down assay, and to activate the expression of transcription factors including ISRE, STAT3 and GAS, in human Janus kinase signal transducer and activator of transcription (JAK/STAT) pathway after co-transfection in HEK-293T cells in the reporter luciferase activity assay. These results suggested that Cg IFNR-3 could bind to Cg IFNLP as an interferon receptor and participate in the activation of JAK/STAT pathway in human, which will benefit for intensive studies of interferon signaling pathway in mollusc. Highlights: Cg IFNR-3 is a cytokine receptor-like 3 molecule identified from Crassostrea gigas . The mRNA of Cg IFNR-3 was dominantly expressed in oyster hemocytes. The expression level of Cg IFNR-3 mRNA in hemocytes was up-regulated by poly (I: C). Cg IFNR-3 could interact with the IFN-like protein of oyster ( Cg IFNLP) in vitro . As a receptor of IFN, Cg IFNR-3 could activate the JAK/STAT pathway in oyster.