Differentially expressed transcripts in stomach of Penaeus monodon in response to AHPND infection. (December 2016)

• Record Type: Journal Article
• Authors: Soonthornchai, Wipasiri; Chaiyapechara, Sage; Klinbunga, Sirawut; Thongda, Wilawan; Tangphatsornruang, Sithichoke; Yoocha, Thippawan; Jarayabhand, Padermsak; Jiravanichpaisal, Pikul
• Is Part Of: Developmental and comparative immunology. Volume 65(2016)
• Page Start: 53
• Page End: 63
• Publication Date: 2016-12
• Subjects: Penaeus monodon -- Vibrio parahaemolyticus -- AHPND -- Stomach -- Ion Torrent sequencing; Immunology -- Periodicals; Developmental immunology -- Periodicals; 616.079
• DOI: 10.1016/j.dci.2016.06.013
• Languages: English
• ISSNs: 0145-305X

Abstract

Abstract: Acute Hepatopancreatic Necrosis Disease (AHPND) is an emerging disease in aquacultured shrimp caused by a pathogenic strain of Vibrio parahaemolyticus . As with several pathogenic bacteria, colonization of the stomach appeared to be the initial step of the infection for AHPND-causing Vibrio . To understand the immune responses in the stomach of black tiger shrimp ( Penaeus monodon ), differentially expressed transcripts (DETs) in the stomach during V . parahaemolyticus strain 3HP (VP3HP ) infection was examined using Ion Torrent sequencing. From the total 42, 998 contigs obtained, 1585 contigs representing 1513 unigenes were significantly differentially expressed with 1122 and 391 unigenes up- and down-regulated, respectively. Among the DETs, there were 141 immune-related unigenes in 10 functional categories: antimicrobial peptide, signal transduction pathway, proPO system, oxidative stress, proteinases/proteinase inhibitors, apoptotic tumor-related protein, pathogen recognition immune regulator, blood clotting system, adhesive protein and heat shock protein. Expression profiles of 20 of 22 genes inferred from RNA sequencing were confirmed with the results from qRT-PCR. Additionally, a novel isoform of anti-lipopolysaccharide factor, Pm ALF7 whose transcript was induced in the stomach after challenge with VP3HP was discovered. This study provided a fundamental information on the molecular response in the shrimp stomach during the AHPND infection that would be beneficial for future research. Highlights: Transcriptome analysis upon Vibrio parahaemolyticus infection of Penaeus monodon was examined. In total, 1585 contigs representing 1513 unigenes were differentially expressed. Expression profiles of 20 of 22 genes from RNA sequencing and qRT-PCR were consonant. A novel isoform of anti-lipopolysaccharide factor, Pm ALF7 was discovered. Its expression level in stomach and lymphoid organs was greater than other tissues.